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Animal studies further demonstrated that sodium acetate intervention (200 mg/kg) improved ovarian function in PCOS model rats by suppressing histone deacetylase (HDAC) activity, evidenced by normalized follicular growth and elevated circulating 17- estradiol levels (77)

Cell Homogenization The pellets containing 3.55 10 6 cells were suspended in 0.1 M phosphate buffer with a pH of 7.5 (Sigma-Aldrich, Darmstadt, Germany), maintaining the proportion of 1 million cells to 0.04 mL of the buffer, then sonicated for 3 5 s at 4 C (Bandelin Sonoplus GM70, Berlin, Germany)